Study on Separation and Purification of Total Saponins from Radix Clematidis with Macroporous Absorptive Resin
- VernacularTitle:大孔吸附树脂分离纯化威灵仙总皂苷的工艺研究
- Author:
Xianxiang XU
;
Lunzhu XIA
- Publication Type:Journal Article
- Keywords:
Radix Clematidis;
Total saponins;
Macroporous absorptive resin
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the separation and purification of total saponins from Radix Clematidis by column chromatography of macroporous absorptive resin. Methods Radix clematidis was extracted with 70 % ethanol and then the extraction was purified with D101 macroporous absorptive resin. After eluting with distilled water and 30 % , 50 % and 70 % ethanol, absorption capability and elution parameters of macroporous absorptive resin were measured by the indexes of elution rate and refinement rate of total saponins. Results Total saponins of Radix Clematidis were enriched in the 50 % ethanol eluted solution fraction. The elution rate of macroporous absorptive resin was 71.26 % and absorption capability was 53.36 mg/g . Conclusion It is feasible to use macroporous absorptive resin for the isolation and purification of total saponins from Radix Clematidis. The optimum elution condition is: removing water- soluble impurity by water, eluting total saponins with 50 % ethanol.
