Inhibition on Mast Cell Degranulation and Histamin Releasing of EtoH Ext. of Roasted Perilla Seed in Vitro
- VernacularTitle:炒紫苏子醇提物对肥大细胞脱颗粒及组胺释放的影响
- Author:
Qinfu WANG
;
Yongqi WANG
;
Chao YU
- Publication Type:Journal Article
- Keywords:
roasted perilla seed;
mast cell;
histamin releasing;
anti-allergic
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the inhibition of the EtOH ext. of roasted perilla seed on mast cell degranulation and histamin releasing in vitro. Methods Aliquots of OVA-sensitized rat peritoneal mast cells (PMC) were preincubated with 320, 640, 12 80 ?g/mL EtOH ext. of roasted perilla seed, disodium cromoglicate and luteolin, before an optimal concentration of OVA were added. Then the PMC degranulation response upon allergen triggering was detected by PMC degranulation percentage and histamine releasing assay. Result The protocol was optimized as PMC were preincubated with drugs for 20 min, and then triggered with 20 ?g/mL OVA. 320, 640, 12 80 ?g/mL EtOH ext. of roasted perilla seed and luteolin could inhibit PMC degranulation significantly (P
