THE EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON PROTEIN KINASE B ACTIVITY
- VernacularTitle:碱性成纤维细胞生长因子对人胚肾HEK293细胞蛋白激酶B活性的影响
- Author:
Huiying YU
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor;
Protein kinase B;
Phosphatidylinositol 3-kinase;
[?-()~(32)P]ATP incorporation assay;
HEK293 cell
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To study the effects of basic fibroblast growth factor(bFGF) on protein kinase B(PKB) activity. Methods The cytosol and membrane activity of PKB at the indicated time periods was determined by [?-()~(32)P]ATP incorporation assay. Results Both membrane and cytosol activity of PKB in HEK293 cell treated with bFGF(75??g/L) reached to the peak at 10?min.Wortmannin efficiently inhibited the activity of PKB in HEK293 cell.Conclusion bFGF rapidly stimulats both membrane and cytosol activity of PKB via P13K in HEK293 cell.
