Effect of spider venom from Macrothele raveni on proliferation and cell cycle of human hepatocellular carcinoma cell line SMMC-7721
- VernacularTitle:雷氏大疣蛛毒素对人肝癌细胞SMMC-7721细胞增殖及细胞周期的影响
- Author:
Jie SUN
;
Li GAO
;
Yongqing SHEN
;
Baoen SHAN
- Publication Type:Journal Article
- Keywords:
spider venom from Macrothele raveni Zhu,Li & Song;
SMMC-7721 cells;
c-myc protein
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of spider venom from Macrothele raveni on proliferation and cell cycles of human hepatocellular carcinoma cell line SMMC-7721 and the molecular mechanism of the(effect.) Methods Proliferation of SMMC-7721 cells was determined by MTT assay.DNA synthesis of SMMC-7721 cell pre-and post-treatment with spider venom from M.raveni was tested by -TdR(assay.) The induction of apoptosis and the change of cell cycle in SMMC-7721 cells treated with spider(venom) from M.raveni were investigated by Flow Cytometry.The effect of spider venom from M.raveni on expression of c-myc protein in SMMC-7721 cells was studied by Western Blot.Results MTT assay showed that the proliferation of SMMC-7721 cells in vitro was inhibited by spider venom from M.raveni((P
