Effect of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation
- VernacularTitle:青藤碱清除氧自由基和抗脂质过氧化作用
- Author:
Gang LIU
;
Hui WANG
;
Xianzhou ZHANG
;
Shunde LUO
- Publication Type:Journal Article
- Keywords:
sinomenine;
oxygen free radical;
lipid peroxidation
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation in vitro. Methods Superoxide anion free radical(O_2~?) induced by the auto-oxidation of pyrogallol and hydroxyl free radical(?OH) induced by Fenton system were determined by spectrophotometry.The inhibition on homogenate and mitochondrial lipid peroxidation,malondialdehyde(MDA),of rat liver induced by VC-Fe~(2+) system was measured by thiobarabituric acid(TBA) colorimetric method.Results Sinomenine could significantly scavenge O_2~? and ?OH,it's EC_(50) were 1.35 and 0.136 mg/mL respectively.Sinomenine could also remarkably inhibit the lipid peroxidation of rat liver homogenate and mitochondria induced by auto-oxidation and VC-Fe~(2+) system.The scavenging and inhibitory effect exhibited in a concentration-dependent manner.Conclusion The results indicate that sinomenine possesses the effect of scavenging oxygen free radicals and anti-lipid peroxidation,which shows the protection of liver mitochondria.
