Lethal effect of hyperthermic CO2 pneumoperitoneum on gastric cancer cells
10.3760/cma.j.issn.1673-4203.2012.07.004
- VernacularTitle:热CO2气腹对胃癌细胞的杀伤作用
- Author:
Jiran ZHANG
;
Daorong WANG
;
Dong TANG
;
Liuhua WANG
;
Jie CHEN
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Apoptosis;
Hyperthermia,induced;
CO2 pneumoperitoneum
- From:
International Journal of Surgery
2012;39(7):440-444,505
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the lethal effect of hyperthermic CO2 pneumoperitoneum on gastric cancer cells and then further study the feasility and safety of inflating hyperthermic CO2 in gastric cancerpatients when performing laparoscopic operation.MethodsAn in vitro hyperthermic CO2 pneumoperitoneum experimental model was built,then according to the experimental purpose,the stomach carcinoma cell-7901 was grouped:hyperthermic CO2 pneumoperitoneum group; pure hyperthermia group; pure CO2 group;control group.After processing according to groups,cell proliferation was detected by Cell Counting Kit-8(CCK-8),cell apoptosis was detected by Annexin V-fluorescein isothiocyanate/propidium iodide flow cytometry and Hoechst 33342/propidium iodide fluorescent microscopy.ResultsThe reaults of cell proliferation detection showed that hyperthermic CO2 pneumoperitoneum could significantly inhibit proliferation of stomach carcinoma cell-7901 when compared with pure hyperthermia group,pure CO2 group and control group (P ＜ 0.05 ).The results of apoptosis detection showed that hyperthermic CO2 pneumoperitoneum could significantly induce apoptosis of stomach carcinoma cell-7901 and the gastric cancer cells apoptosis showing bright blue under the fluorescent microscope.ConclusionsHyperthermic CO2 pneumoperitoneum could significantly inhibit proliferation of stomach carcinoma cell-7901 and probably kill it by inducing apoptosis.
