Effect of Chai Qin Pingwei Capsule on Gastric Mucosal Cell Apoptosis and Regulatory Genes in Rats with Bile Reflux Gastritis
- VernacularTitle:柴芩平胃胶囊对反流性胃炎胃粘膜细胞凋亡及调控基因的影响
- Author:
Hong YANG
;
Jiayu HOU
;
Delu TIAN
- Publication Type:Journal Article
- Keywords:
CHAI QIN PINGWEI CAPSULE/pharmacology;
GASTRITIS/TCD therapy;
CELL APOPTOSIS;
GENE EXPRESSION REGULATION;
DISEASE MODELS, ANIMAL;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effect of Chai Qin Pingwei Capsule (CQPC) on gastric mucosal cell apoptosis and regulatory genes in rats with bile reflux gastritis. [ Methods ] Wistar rats were randomized into 6 groups: sham-operation group (A), model group (B) , CQPC groups in the dosages of 16.66 (high) ,8.33 (moderate), and 4.17 (low) g/kg respectively (C, D and E respectively), Xiao Chaihu Granules group in the dosage of 10g/kg (F). Except the sham-operation group, the rats in other groups received B- II gastrojejunostomy to induce bile reflux gastritis and were treated with gastric gavage of corresponding drugs according to the experimental design for 4 weeks. Effects of CQPC on gastric mucosal cell apoptosis and the expression of p53 mRNA, Bax and Bcl-2 were observed by enzyme-linked immunosorbent assay (ELISA), hybridization in situ and immunohistochemistry method. [Results] Bile reflux in the model group caused the increase of gastric mucosal cell apoptosis, the up-regulation of wild-type p53 mRNA and Bax protein expression, and the down-regulation of Bcl-2 protein expression, the difference being significant as compared with the sham-operation group (P
