HPLC Determination of Magnolin in Xuetie Dingchuan Plaster
- VernacularTitle:HPLC法测定穴贴定喘膏中木兰脂素的含量
- Author:
Meihua NI
;
Liuqing DI
;
Shuping SUN
- Publication Type:Journal Article
- Keywords:
Xuetie Dingchuan Plaster;
Magnolin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of magnolin in Xuetie Dingchuan Plaster.Methods The HPLC chromatographic condition was as follows: the analytical column was Lichrospher C8(4.6mm?250mm,5?m),the mobile phase consisted of A(acetonitrile)∶B(1.55%tetrahydrof uran-water) 34∶66,the flow rate was 1.0 mL?min-1,the detected wav elength was 278 nm,and the column temperature was 30 ℃.Results The calibr ation curve was linear(r=0.9999) in the range of 0.42～ 5.25 ?g for magnolin,the average recovery of the method was 100.50 %,with RSD 2.22 %(n=9).Conclusion The method is accurate,simple and stable,and can be used for the quality control of Xuetie Dingchuan Plaster.
