Determination of Gastrodine Content in Tianma Formula Granule by HPLC
- VernacularTitle:HPLC法测定天麻配方颗粒中天麻素的含量
- Author:
Jun WAN
;
Xia ZHOU
;
Wenwen CHEN
;
Chunjie WU
- Publication Type:Journal Article
- Keywords:
Tianma Formula Granule;
Gastrodine;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of gastrodine in Tianma Formula Granule.Methods A HPLC was performed on Shim-pack VP-ODS(4.6mm? 150mm,5? m).The mobile phase consisted of acetonitrile and 0.1 % H3PO4(3∶ 97).Detected wavelength was at 220 nm,the flow r ate was 0.5 mL? min-1,column temperature was at 25 ℃,and the injection volume was 5 ? L.Results The linearity was good in the range of 0.1020～0. 6120 ? g for gastrodine(r=0.9999).The average recovery rate was 100.5 %, and RSD was 1.01 %(n=5).Conclusion The established HPLC method is simple,accurate,and suitable for the determination of gastrodine in Tianma Formula Granule.
