Quantitative Analysis of Diosgenin in Jingangteng Soft Capsule by RP-HPLC
- VernacularTitle:RP-HPLC法测定金刚藤软胶囊中薯蓣皂苷元的含量
- Author:
Juan SHEN
;
Xiangfeng WU
;
Zhenyu ZHU
;
Yifeng CHAI
- Publication Type:Journal Article
- Keywords:
Jingangteng soft capsule;
Diosgenin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine diosgenin content in Jingangteng soft capsule by RP-HPLC.Methods A RP-HPLC was performed on a Shim-Pak VP-ODS C18column(5?m,4.6 mm? 250 mm),the mobile phase consisting of acetonitri le-H2O(87∶13) and the detected wavelength at 209 nm.Results A good line arity was obtained in the range 0.0595～ 14.875 ? g(r=0.99999) for diosgenin. The average recovery and RSD were 99.4 % and 2.0 % respectively(n=5).Concl usion The method is simple,rapid,stable and reliable,and can be used to determine diosgenin content in Jingangteng soft capsule.
