Determination of Oxymatrine in Oxymatrine Injection by HPLC
- VernacularTitle:HPLC法测定苦参素注射液中苦参素的含量
- Author:
Zhenghong WU
;
Baiyang MAO
;
Yongjun WANG
- Publication Type:Journal Article
- Keywords:
Oxymatrine Injection;
oxymatrine;
HPLC;
content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for the determination of oxymatrine in Oxymatrin Injection.Methods A RP-HPLC method with Lichrospher 5 ODS C18 column(4.6 mm? 250 mm,5 ? m) was used,and the mobile phase was the mixture of acetonnitrile-pH2.0 sulphate buffer solution(7∶93) at the f low rate of 1.0mL/min.The column temperature was 30 ℃,the UV detection wave length was 220 nm and the injection volume was 20 ? L.Results The calibra tion curve was linear(r=1.0000) in the range of 3.63～ 116.04 ? g/mL for oxyma trine.The mean recovery was 100.1 %(RSD=0.86 %,n=6).Conclusion Thi s method is effective and can be used for the determination of oxymatrine in Oxymatrine Injection.
