Laboratory Identification of Leptotrichia Species Isolated From Bacteremia Patients at a Single Institution.
10.3343/alm.2017.37.3.272
- Author:
Eun Hye CHO
1
;
Kyung Sun PARK
;
Mina YANG
;
Dong Joon SONG
;
Hee Jae HUH
;
Chang Seok KI
;
Nam Yong LEE
Author Information
1. Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. pmhhj77@gmail.com micro.lee@samsung.com
- Publication Type:Brief Communication
- Keywords:
Leptotrichia;
Bacteremia;
Identification;
MALDI-TOF MS;
16S ribosomal RNA
- MeSH:
Bacteremia*;
Humans;
Leptotrichia*;
Mass Spectrometry;
RNA, Ribosomal, 16S
- From:Annals of Laboratory Medicine
2017;37(3):272-276
- CountryRepublic of Korea
- Language:English
-
Abstract:
We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level. Species level identification was successfully achieved by using 16S ribosomal RNA gene sequencing.
