Determination of matrine and phillyrin in Jieyinjing Suppositories by HPLC
- VernacularTitle:HPLC法测定洁阴净栓中苦参碱和连翘苷的含量
- Author:
Yanling LI
- Publication Type:Journal Article
- Keywords:
Jieyinjing Suppositories;
matrine;
phillyrin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the content determination method of matrine and phillyrin in Jieyinjing Suppositories(Radix Sophorae Flavescentis,Fructus Forsythiae,Cortex Phellodendri Chinensis,Radix Stemonae,etc.) by HPLC. METHODS: HPLC was performed to determine matrine on ODS column,the mobile phase consisting of methanole-0.02% triethylamine(55∶45) and detection wavelength at 220 nm and the phillyrin of mobile phase consisting of acetonitrile-water(25∶75) and its detection wavelength at 277 nm. RESULTS: The linearity of matrine was in the range of 9.8 ?g?mL~(-1)-49.0 ?g?mL~(-1)(r=0.999 96),and the average recovery was(99.48%,) RSD=1.49%;The linearity of phillyrin was in the range of 5.4 ?g?mL~(-1)-54.0 ?g?mL~(-1)(r=(0.999 9),) and the average recovery was 100.07%,RSD=1.34%. CONCLUSION: This method is simple,sensitive and accurate,and available for control of Jieyinjing Suppositories.
