Ceramide mechanism of anti-myocardial ischemia/reperfusion injury of effective position in Sini Decoction
- VernacularTitle:四逆汤有效部位抗心肌缺血/再灌注损伤神经酰胺机制的研究
- Author:
Huilan SUN
;
Weikang WU
;
Hanchuan LUO
;
Tianwen LIANG
- Publication Type:Journal Article
- Keywords:
Sini Decoction's effective component(SNDE);
ceramide;
myocardial ischemia/reperfusion;
apoptosis
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of Sini Decoction's effective component(SNDE) on myocardial apoptosis and ceramide content during myocardial ischemla/reperfusion. METHODS: The mice of Kunming species were randomly divided into 3 groups: control,myocardial ischemia/reperfusion and SNDE.After inducing myocardial ischemia/reperfusion injury of mice in vivo with pituitrin(Pit),myocardial SOD activity and MDA content were measured.DNA agarose gel electrophoresis and fluorescent stain of DAPI were done to check up apoptosis.The content of myocardial ceramide(?g/kg) was measured through HPTLC and scan of thin plate. RESULTS: The myocardium of model group has the phenomenon of DNA ladder.The apoptosis index and the ceramide content in model group were higher than those of control group(P
