Study on the relation between concentration of circulating non-host fetal DNA in pregnant women and pre-eclampsia
- VernacularTitle:孕妇外周血中胎儿DNA水平检测在子痫前期诊断中的应用
- Author:
Hong TAO
;
Xuemei WANG
;
Xianghong JI
- Publication Type:Journal Article
- Keywords:
DNA;
Pre-eclampsia;
Polymerase chain reaction;
Endothelins
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the diagnostic value of plasma fetal DNA level in preeclampsia.Methods Thirty cases of pregnant women with preeclampsia(at 33 weeks and 3 days) and 30 cases of normal pregnant women(at 34 weeks and 3 days) were selected.All the pregnant women carried a male fetus by B-ultrasound,and were sampled at gestational 20 weeks,third trimester and at 1 hour,3 hours,6 hours after delivery.SRY levels in maternal blood were quantitated by polymerase chain reaction(QF-PCR).The endotheliotoxin(ET) level was measured with RIA.Results (1) Mean fetal DNA level of patients with preeclampsia at 20 weeks of gestation was(316?61)copy/ml.They were(266?79)copy/ml,(396?91)copy/ml,(165?43) copy/ml for light and severe preeclampsia women and normal pregnant women,respectively.Maternal blood fetal DNA levels in pregnant women with preeclampsia at 20-weeks of gestation were significantly higher than those normal pregnant women(P
