In vitro and vivo experimental study on ameliorating lung damage after cardiopulmonary bypass by acceleration neutruphils apoptosis
- VernacularTitle:诱导中性粒细胞凋亡减轻体外循环后肺损伤的体内外实验研究
- Author:
Xiangming FAN
;
Yinglong LIU
;
Qiang WANG
- Publication Type:Journal Article
- Keywords:
Apoptosis Extracorporeal circulation Lung Reperfusion injury
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of acceleration neutruphils apoptosis on lung injury after cardiopulmonary bypass(CPB) . Methods The neutrophils were separated using discontinuous Percoll gradients in vitro and cultured for 48 hours with or without clarithromycin. Neutrophil survival was determined by typan blue dye exclusion method. Apoptotic neutrophils were detected with flow cytometry. Immune histochemistry was used to examine the expression of protein of Fas and bcl-2 which interrelated with neutrophil apoptosis. In vivo study twelve adult sheeps were randomly divided into two groups. Pulmonary artery was clamped after CPB was established and the lungs were perfused with 4℃ low molecular weight dextran solution in control group (n = 6) and 4℃ low molecular weight dextran solution with clarithromycin in protective group (n - 6), The bypass was withdrawn after 90 minutes. Respiratory function were recorded during CPB and cytokines in the lung were measured. Lung biopsies and the apoptosis of the neutrophil were also performed after operation. Results Clarithromycin significantly shortened neutrophil survival in a dose-dependent fashion. The result of flow cytometry showed that the apoptosis rates at 24h of clarithromycin group were (33.7?4.9)%, (48.0?4.9)%, (52.0?5.4)% and (53.0?7.1)% at 1, 5, 10 and 20 ?g/ml of clarithromycin respectively while the rate in control group was (31.5?3.5)% (P
