Accelerated solvent extraction of ecdysterone from Achyranthes bidentata
- VernacularTitle:牛膝蜕皮甾酮的加速溶剂提取法研究
- Author:
Jiaquan WANG
;
Minjun ZHAO
;
Lan LIN
;
Yinglei CHAI
;
Su ZENG
- Publication Type:Journal Article
- Keywords:
Achyranthes bidentata Bl.;
ecdysterone;
accelerated solvent extraction (ASE);
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply accelerated solvent extraction (ASE) technique to extract Achyranthes bidentata and to explore the application of this technique in quality control of Chinese materia medica. Methods Investigation of single factor was used to optimize the conditions that affected the efficiency for ASE from A. bidentata by RP-HPLC using ecdysterone as a quantitative marker. Results The optimized conditions for ASE of A. bidentata were obtained as follows: methanol as solvent, particle size between 0.3 and 0.45 mm, temperature at 100 ℃, pressure under 10.34 MPa, 6 min duration and once extraction. Conclusion ASE technique can be used to extract A. bidentata quickly and effectively.
