Effects of extract from Narcissus tazettavar. chinensis on proliferation and apoptosis of human multiple myeloma cell line ARH-77
- VernacularTitle:水仙提取物对人类多发性骨髓瘤细胞系ARH-77增殖和凋亡的作用
- Author:
Jing LIU
;
Weixin HU
;
Lifang HE
- Publication Type:Journal Article
- Keywords:
the extract from Narcissus tazetta var. chinensis Roem.;
human multiple myeloma;
cell proliferation;
cell apoptosis
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of extract from Narcissus tazetta var. chinensis on proliferation and apoptosis of human multiple myeloma cell line ARH-77. Methods The survival rate of cells were tested by MTT assay when cells were affected by 1.25, 2.5, 5, 10, 20 ?g/mL of the extract for 3 d. After being induced by 10 ?g/mL of the extract for 3 d, apoptotic cells were observed with fluorescence stain; changes of he csll cycle were analyzed by Flow Cytometry; the number and shape of nucleolar organizer region (NOR) were tested by AgNOR assay and submicroscopic structure were observed by transmission electron microscope. Results The extract from N. tazetta var. chinensis could significantly inhibit the proliferation and reduce the survival rate of ARH-77 cells (P
