NT-3 GENETICALLY MODIFIED SCHWANN CELLS PROMOTE GRAFTED NEURAL STEM CELLS DIFFERENTIATE INTO NEURON-LIKE CELLS IN THE INJURED SPINAL CORD
- VernacularTitle:NT_3基因修饰施万细胞促进移植的神经干细胞在损伤脊髓内分化为神经元样细胞
- Author:
Jiasong GUO
- Publication Type:Journal Article
- Keywords:
Neural stem cells;
Neurotrophin-3;
Genetically modified Schwann cells;
Differentiation;
Neuron-like cells;
Spinal cord injury;
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To explore the effects of Neurotrophin-3(NT-3) genetically modified Schwann cells(SCs) on grafted neural stem cell(NSC) differentiation into the neuron-like cells in the transected spinal cord. Methods The NSCs were single-transplanted or co-engrafted with NT-3 modified SCs,report gene LacZ modified SCs,and unmodified SCs respectively into the transected spinal cord 67d later,the differentiation of NSCs was studied by immunohistochemisty,and the percentage of neuron-like cells was calculated. Results NSCs could differentiate into the neuroglial-like cells and neuron-like cells in the injured spinal cord.Compared to the unmodified SCs,NT-3 modified SCs could more efficiently promote NSCs differentiate into the neuron-like cells.The effect of LacZ modified SCs was the same to the unmodified SCs.Conclusion NT-3 modified SCs could promote NSCs differentiate into the neuron-like cells in the injured spinal cord.
