EXPRESSION OF GAL?-1,4-GlcNAc GROUP IN ASTROCYTOMA
- VernacularTitle:?-1,4-半乳糖苷键在星形胶质细胞瘤中的表达特征
- Author:
Meijuan YANG
- Publication Type:Journal Article
- Keywords:
Gal?-1,4-GlcNAc group;
Ricinus communis agglutinin-I(RCA-I);
Astrocytoma;
RCA-I lectin blot;
Human
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To study the expression of Gal?-1,4-GlcNAc group in human normal brains and astrocytomas. Methods The protein extracts from human normal brains and astrocytomas were subjected to sodium dodecyl sulfate polyacrylamide gel electropheresis(SDS-PAGE),then were stained with Coomassie Brilliant blue(CBB).In addition,the expression of Gal?-1,4-GlcNAc group was studied by ricinus communis agglutinin-I(RCA-I) lectin blot and lectin histochemistry with RCA-I respectively. Results By comparison,CBB staining showed that the protein components from normal brains were similar to those from astrocytomas.However,by RCA-I lectin blot and histochemistry assay,it was found that the expression of Gal?-1,4-GlcNAc was more extensive in astrocytomas than normal brains.It was surprising that a major 61?kD protein was galactosylated in astrocytomas but not in normal brains.Ratio of RCA-I stained cells was 8% in human normal brains,while 15%,21%,28% and 41% in astrocytomas Ⅰ,Ⅱ,Ⅲ and Ⅳ respectively.Conclusion The expressions of Gal?-1,4-GlcNAc groups in astrocytomas were more than those in normal brains,which suggested that Gal?-1,4-GlcNAc group might be related to the malignant degree of astrocytomas.
