ORIENTATIONAL DEVELOPMENT AND DIFFERENTIATION OF MOUSE PRIMORDIAL GERM CELLS INTO HEPATOCYTES IN VITRO
- VernacularTitle:定向诱导小鼠原始生殖细胞向肝细胞分化的研究
- Author:
Xiaoping LIU
- Publication Type:Journal Article
- Keywords:
Primordial germ cells(PGCs);
Hepatocyte;
?-NGF;
bFGF;
EGF;
RA;
Differentiation;
Mouse
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the better factor for inducing the primordial germ cells to differentiate into hepatocytes in vitro. Methods The primordial germ cells(PGCs) from the gonadal ridges of the mouse embryos at 11 days postcoitum(dpc) from Kunming pregnancy mice were cultured and proliferated in vitro.An aliquot PGCs suspension was cultured in 6-well dishes for 24?h.Then bFGF, EGF,?-NGF,RA and hepatocyte abstract were added into the dishes respectively,Anather aliquot PGCs were co-cultured with mouse embryonic hepatocytes for directional differentiation.The morphological changes and indiocyanine green(ICG) intake of the PGCs were observed;albumin(ALB) and ?-1-antitrypsin(AAT) were assayed by immunocytochemistry. Results After the addition of ?-NGF,hepatocyte abstract and CM-EH the ICG was intaken by the differentiated cells which like star and ovum,the ALB and AAT immune positive expression were detected in those differentiated cells.The ratio of positive cells was above 60% in 2 weeks.But bFGF,EGF and RA had no same as the effects.However RA could improve the ratio of differentiated cells induced by ?-NGF and hepatocyte abstract.Conclusion ?-NGF and some factors from hepatocytes and embryonic hepatocytes could effectively induce PGCs to differentiate into hepatocytes.
