THE EXPRESSION OF LAMININ IN THE DEVELOPING KIDNEY OF MOUSE
- VernacularTitle:小鼠肾发育中层黏连蛋白表达的实验研究
- Author:
Min GUO
- Publication Type:Journal Article
- Keywords:
Laminin;
Developing;
Kidney;
Immunohistochemistry;
Mouse
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether laminin(LN) is expressed in the developing kidney of mouse and the changes of the expression associated with maturation and polarity. Methods Eight fetal and fourteen postnatal kidneys were examined by immunohistochemistry(Strepavidin Peroxidase conjugated method,SP),using polyclonal rabbit anti-mouse antibody. Results The expression of laminin in the kidneys increased with the gestational weeks of fetus and maturity of the kidney.The expression of laminin was positive in monocell,multicell without space and multicell with space.In the developing kidney,the laminin is expressed in the ureteric bud,glomerular basement membrane(GBM),tubular basement membrane(TBM),renal capsular basement membrane and interstitium.The expression of TBM was prior to the expression of GBM.Conclusion The expression of laminin in the developing kidney of mouse has chronological and spatial sequence.The laminin determines the polarity of cell.Laminin might play a key role during nephrogenesis.
