In Vitro and in Vivo Effects of Nitrofurantoin on Experimental Toxoplasmosis.
10.3347/kjp.2016.54.2.155
- Author:
Seon Ju YEO
1
;
Chunmei JIN
;
Sungyeon KIM
;
Hyun PARK
Author Information
1. Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, Korea. hyunpk@wku.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Toxoplasma gondii;
nitrofurantoin;
selectivity;
therapeutic efficacy;
mouse;
HeLa cell
- MeSH:
Alanine Transaminase;
Animals;
Aspartate Aminotransferases;
Drug-Related Side Effects and Adverse Reactions;
Female;
Glutathione;
HeLa Cells;
Hematology;
Humans;
Liver;
Malondialdehyde;
Mice;
Mice, Inbred ICR;
Nitrofurantoin*;
Peritoneal Cavity;
Pyrimethamine;
Sulfadiazine;
Toxoplasma;
Toxoplasmosis*
- From:The Korean Journal of Parasitology
2016;54(2):155-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toxoplasma gondii is an important opportunistic pathogen that causes toxoplasmosis, which has very few therapeutic treatment options. The most effective therapy is a combination of pyrimethamine and sulfadiazine; however, their utility is limited because of drug toxicity and serious side effects. For these reasons, new drugs with lower toxicity are urgently needed. In this study, the compound, (Z)-1-[(5-nitrofuran-2-yl)methyleneamino]-imidazolidine-2,4-dione (nitrofurantoin), showed anti-T. gondii effects in vitro and in vivo. In HeLa cells, the selectivity of nitrofurantoin was 2.3, which was greater than that of pyrimethamine (0.9). In T. gondii-infected female ICR mice, the inhibition rate of T. gondii growth in the peritoneal cavity was 44.7% compared to the negative control group after 4-day treatment with 100 mg/kg of nitrofurantoin. In addition, hematology indicators showed that T. gondii infection-induced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, biochemical parameters involved in liver injury, were reduced by nitrofurantoin significantly. Moreover, nitrofurantoin exerted significant effects on the index of antioxidant status, i.e., malondialdehyde (MDA) and glutathione (GSH). The nitrofurantoin-treated group inhibited the T. gondii-induced MDA levels while alleviating the decrease in GSH levels. Thus, nitrofurantoin is a potential anti-T. gondii candidate for clinical application.
