The study of indirect osteoclast differentiation effect of LTB4 in rheumatoid arthritis
- VernacularTitle:类风湿关节炎中白三烯B4间接分化破骨细胞的实验研究
- Author:
Jun JIANG
;
Houshan LU
;
Jianhao LIN
;
Zhenpeng GUAN
;
Jian HUANG
- Publication Type:Journal Article
- Keywords:
Receptors, Leukotriene B4;
Arthritis, rheumatoid;
Osteoclasts;
Differentiation
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether LTB4 could indirectly stimulate human osteoclast differentiation in RA through increasing RANKL expression of RAFLs. Methods We utilize the coculture model of RAFLs and monocyte which were stimulated in the presence of 2.5 ng/ml M-CSF in the control group, 2.5 ng/ml M-CSF +10-8 mol/L LTB4 in the experimental group A, 2.5 ng/ml M-CSF+10-8 mol/L LTB4+100 ng/ml OPG in the experimental group B. After culture for 3 weeks, through TRAP staining we counted the number of multinucleated TRAP staining positive osteoclast-like cells stained with TRAP to evaluate the differentiation effect in each group. Results There was almost no osteoclast-like cell in the control group and the experimental group B. Whereas there were many osteoclast-like cells in the experimental group A. Conclusion LTB4 can indirectly stimulate human osteoclast differentiation in RA through increasing RANKL expression of RAFLs.
