Protective effects of carboxymethyl-chitosan on the proliferation and phenotype of interleukin-1?-in-duced chondrocytes
- VernacularTitle:羧甲基壳聚糖对白细胞介素-1?刺激的软骨细胞增殖能力和表型的保护作用
- Author:
Shiqing LIU
;
Qing CHEN
;
Yuming DU
;
Hao PENG
;
Liping SUN
- Publication Type:Journal Article
- Keywords:
Chitosan;
Interleukin-1;
Chondvocyte;
Nitric oxide
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of carboxymethyl-chitosan (CM-chitosan) on phenotype and proliferation of chondrocytes stimulated by recombinant human interleukin-1? (rhIL-1?), and explore its mechanism. Methods Chondrocytes were isolated and cultured. 10 ng/ml IL-1? with or without CM-chitosan of varied concentrations were added into the culture medium. After 24 h, changes of proliferative ability of chondrocytes were tested by MTT and flow cytometry. Proteoglycan synthesis was measured by incorporation of Na235SO4 into chondrocytes, nitric oxide (NO) production was detected by Greiss reaction, mRNA expression of typeⅠ、Ⅱcollagens, Aggrecan and inducible NO synthase (iNOS) were examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results CM-chitosan could significantly antagonize IL-1?-induced inhibitory effect on proliferation of chondrocytes, restore proteoglycan synthesis of chondrocytes, decrease NO production of IL-1?-induced chondrocytes, increase the mRNA expression of typeⅡcollagen and Aggrecan, but decrease the expression of typeⅠcollagen and iNOS. The effects of CM-chitosan were in a dose-dependent manner. Conclusion CM-chitosan can maintain the proliferation and phenotype of IL-1?-induced chondrocytes.
