Effects of natural killer T cells activated by ?-galactosylceramide on BWF1 mice
- VernacularTitle:自然杀伤T细胞活化对狼疮鼠(NZB?NZW)F1的影响
- Author:
Guosheng WANG
- Publication Type:Journal Article
- Keywords:
Lupus erythmatosus, systemic;
Mice;
T-lymphocytes;
Cerebrosides
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To explore the effects of natural killer (NK) T cells activated by ?-galactosylceramide (?-GalCer) on (NZB?NZW) F1 mice (BWF1). Methods Forty BWF1 mice were divided into two groups (5th week group, 6th month group) according to the age when the treatment started. Mice were treated with ?-GalCer or vehicle by intraperitoneal injection of various dosages and therapeutic courses. The survival time and proteinuria were recorded. Mice blood were withdrawn before and every month after injection, and the collected sera were frozen for analysis of IgG anti-dsDNA, anti-cardiophospholipid antibodies (ACL) and rheumatoid factor (RF). Another ten mice′s blood were withdrown 2 h later after ?-GalCer or vehicle iv injection. Cytokines including IL-2, IFN-?, IL-4 and IL-10 were detected. Results Cytokines IL-2 and IFN-? secreted by Th1 were increased while cytokines IL-4 and IL-10 secreted by Th2 decreased in ?-GalCer iv injection mice. The level of IgG anti-dsDNA antibodies and RF increased with age in ?-GalCer treated mice which was lower than those of vehicle treated mice. No difference in survival rate and severe proteinuria (≥3+) rate was observed between ?-GalCer and vehicle treated BWFI mice in the two groups. Conclusion ?-GalCer may activate NKT cells and decrease some autoantibodies. NKT cells activation using ?-GalCer is not enough to improve proteinuria and survival rate.
