Complete Mitochondrial Genome of Echinostoma hortense (Digenea: Echinostomatidae).
10.3347/kjp.2016.54.2.173
- Author:
Ze Xuan LIU
1
;
Yan ZHANG
;
Yu Ting LIU
;
Qiao Cheng CHANG
;
Xin SU
;
Xue FU
;
Dong Mei YUE
;
Yuan GAO
;
Chun Ren WANG
Author Information
1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, P. R. China. chunrenwang@sohu.com
- Publication Type:Original Article
- Keywords:
Echinostoma hortense;
complete mitochondrial DNA;
echinostomatidae;
phylogenetic analysis
- MeSH:
Amino Acid Sequence;
Base Sequence;
Databases, Nucleic Acid;
Dataset;
Echinostoma*;
Echinostomatidae;
Fasciolidae;
Genes, rRNA;
Genetic Markers;
Genetics, Population;
Genome;
Genome, Mitochondrial*;
Humans;
RNA, Transfer;
Trematoda
- From:The Korean Journal of Parasitology
2016;54(2):173-179
- CountryRepublic of Korea
- Language:English
-
Abstract:
Echinostoma hortense (Digenea: Echinostomatidae) is one of the intestinal flukes with medical importance in humans. However, the mitochondrial (mt) genome of this fluke has not been known yet. The present study has determined the complete mt genome sequences of E. hortense and assessed the phylogenetic relationships with other digenean species for which the complete mt genome sequences are available in GenBank using concatenated amino acid sequences inferred from 12 protein-coding genes. The mt genome of E. hortense contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 non-coding region. The length of the mt genome of E. hortense was 14,994 bp, which was somewhat smaller than those of other trematode species. Phylogenetic analyses based on concatenated nucleotide sequence datasets for all 12 protein-coding genes using maximum parsimony (MP) method showed that E. hortense and Hypoderaeum conoideum gathered together, and they were closer to each other than to Fasciolidae and other echinostomatid trematodes. The availability of the complete mt genome sequences of E. hortense provides important genetic markers for diagnostics, population genetics, and evolutionary studies of digeneans.
