Determination of 2,3,5,4′-Tetrahydroxystilbene-?-O-?-D-Glucoside in Radix polygoni multiflori preparata by HPLC
- VernacularTitle:HPLC测定制首乌药材中羟基芪衍生物的含量
- Author:
Yun LIU
;
Dong ZHANG
;
Hua YUAN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
2,3,5,4′-tetrahydroxystilbene-?-O-?-D-glucoside;
Radix polygoni multiflori preparata
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish HPLC for determination of 2,3,5,4′tetrahydroxystilbene-?-O-?-D-glucoside in Radix polygoni multiflori preparata. METHODS: The column was diamonsil C_(18)(5 ?m,4.6 mm?250 mm).The mobile phase was CH_3CN-CH_3OH-H_2O(10∶20∶70) and ?=320 nm.The flow rate was 1.5 ml/min. RESULTS: The linearity was in the range of 0.0253-0.3542 ?g.The average recovery and RSD were 99.34% and 0.49%,respectively. CONCLUSION: The method is simple,precise and reproduciable and can be used for determination of 2,3,5,4′-tetrahydroxystilbene-?-O-?-D-glucoside in Radix polygoni multiflori preparata effectively.
