The biological effects of low intensity pulsed ultrasound on human periosteal cells and marrow stromal cells in vitro
- VernacularTitle:低强度脉冲超声波刺激对人骨髓基质细胞和骨膜细胞生物学效应的影响
- Author:
Chao ZHANG
;
Guosui LIANG
;
Yingkai ZHANG
;
Yunyu HU
;
Dike RUAN
- Publication Type:Journal Article
- Keywords:
Low intensity pulsed ultrasound;
Periosteum;
Bone marrow stromal cell;
Cell culture
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological effects of low intensity of pulsed ultrasound (LIPUS) on human periosteal cells and marrow stromal cells in vitro. Methods LIPUS of 30 mW/cm2 was used to stimulate the cultured human periosteal cells and marrow stromal cells for 5 min, 10 and 20 min per day, respectively, followed by the examination of cell proliferation and differentiation potential. Results Thymidine incorporation and total protein assay indicated that LIPUS had no significant effect on DNA and protein synthesis of the periosteal and marrow stromal cells, suggesting that LIPUS did not promote proliferation of these cells. However, ALP activity and osteocalcin secretion of the human periosteal cells were dramatically increased. Conclusion The results suggested that low intensity pulsed ultrasound stimulated periosteal cell proliferation and differentiation toward osteogenic lineage, this might contribute to the curing effect of low intensity pulsed ultrasound for fracture.
