Determination of tricin in malt by HPLC
- VernacularTitle:HPLC法测定大麦芽中麦黄酮
- Author:
Junhong LING
;
Nan WANG
;
Yuzhen REN
;
Longhu WANG
;
Jinhui WANG
;
Xian LI
- Publication Type:Journal Article
- Keywords:
malt;
tricin;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for the quantitative analysis of tricin in malt. Methods HPLC method was used. Analysis was performed on Spherigel C_ 18 column (150 mm?4.6 mm, 5 ?m) with acetonitrile-water-tetrahydrofuran-methanoic acid (26∶74∶10∶1) as mobile phase. The detection wavelength was 350 nm, flow rate was 1.0 mL/min, and the analysic volumn was set at 20 ?L. Results The calibration curve of tricin was linear in the range of 0.22-2.15 mg/L (r=0.999 8). The average recovery rate of tricin was 97.4% (RSD=1.7%) (n=9). Conclusion The method can be used to determine tricin in raw malt quantitatively.
