Determination of Berberine Hydrochloride in Weichangning Granules by HPLC
- VernacularTitle:HPLC法测定胃肠宁颗粒剂中盐酸小檗碱含量
- Author:
Cheng YUAN
;
Hua WANG
;
Fangfang ZENG
- Publication Type:Journal Article
- Keywords:
Weichangning granules;
Berberine hydrochloride;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of berberine hydrochloride in Weichangning granules.Methods RP-HPLC method was set up,using Merck-LiChrospher RP-18 column(4 mm ? 250 mm,5?m);the mobile phase was methnd-water(including 0.18 % phosphoric acid and 0.22 % sodium laurylsulfonate)(68 ∶ 32),flow rate being 1.0 mL / min;column temperature was 35 ℃ and the detection wavelength was 345nm.Results A good linearity was obtained in the range of 0.30 ~ 1.80 ?g(r = 0.9995).The average recovery was 98.32 %,with RSD 0.83 %.Conclusion The method is simple,rapid and with good reproducibility for the determination of berberine hydrochloride in Weichangning granules.
