Determination of 5-O-Methylvisamminol in Shufeng Oral Liquid by HPLC
- VernacularTitle:HPLC法测定舒风口服液中5-O-甲基维斯阿米醇苷的含量
- Author:
Qiang XU
;
Shudan ZHANG
;
Ying FENG
- Publication Type:Journal Article
- Keywords:
5-O-methylvisamminol;
Shufeng oral liquid;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of 5-O-methylvisamminol in Shufeng oral liquid.Methods The sample was extracted with methyl and HPLC method was used.The chromatographic conditions were as follows: Alltima-C18 column(150 mm ? 4.6 mm,5 ?m)with a mobile phase of methanol-water(41∶59),the detection wavelength at 293 nm and the flow rate being 1.0mL?min-1.Results A linearity was obtained from 0.12 ?g to 0.84 ?g of 5-O-Methyvisammioside in Shufeng oral liquid with a good correlation(r = 0.99998,n = 7).The average recovery was 99.8 % and RSD = 2.05 %(n = 5).Conclusion This method for determination of 5-O-Methyvisammioside in Shufeng oral liquid is simple,sensitive,specific and accurate.
