Effects of anti-human augmenter of liver regeneration monoclonal antibody on proliferation of HepG2 cells in vitro
- VernacularTitle:抗人肝再生增强因子单克隆抗体对人肝癌细胞株HepG2增殖活性的影响
- Author:
Lin TANG
- Publication Type:Journal Article
- Keywords:
Human augmenter of liver regeneration;
Monoclonal antibody;
Hepatocellular carcinoma;
Cell line
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the existence of expression of human augmenter of liver regeneration(hALR) in HepG2 cells,and to observe proliferation of HepG2 cells after neutralizing hALR with anti-hALR McAb.Methods:the expression of hALR in HepG2 cells was observed immunocytochemistry and reverse transcriptive PCR(RT-PCR).Proliferation of HepG2 cells after neutralization was detected by ~3?H-TdR incorporation approach.Results:① hALR was expressed by HepG2 cells.② Anti-hALR McAb inhibited partially the autonomous growth of HepG2 cells.Conclusion:Anti-hALR McAb inhibit the autonomous growth of hepatoma cells partially;moreover,hALR maintain the autonomous growth of hepatoma cells in vitro through autocrine mechanism.
