Proliferation inhibition and apoptosis induction of cultured vascular smooth muscle cells after ultrasound exposure
- VernacularTitle:超声辐照对血管平滑肌细胞增殖和凋亡的影响
- Author:
Junxia ZHANG
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle cells;
Ultrasound;
Proliferation;
Apoptosis
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of ultrasound exposure(USE) on proliferation and apotosis of vuscalur smooth muscle cells(VSMCs) and to screen out the optimum parameters of USE and(to)discuss its mechanism.Methods:Ultrasound device was made of mono-source of piezoelectric ceramics.Guided by the result of Trypan Blue cell count,the optimum exposure dosage was determined by MTT cell growth curve in 5 days.BrdU incorporation was used to show changes of S phase DNA synthesis after USE.Apoptosis was detected using ELISA 4 hours later after USE.Results:USE had direct cytotoxic effect on cultured VSMCs. The effect was intensified as the duration or intensity increased.At the same duration and intensity level,the effect was stronger in lower frequency group.Growth curves in 5 days were flattened in USE groups especially in 0.705MHz,0.5w/cm~2,5min exposure.BrdU% results indicated that with 0.705MHz,0.5W/cm~2,5min USE,BrdU% against control was 40.5?6.8% vs 55.8?3.3%,P
