Bone marrow mesenchymal stem cells to express cardiogenic phenotype in a coculture system
- VernacularTitle:在心肌细胞/MSCs共培养体系中MSCs出现心肌分化表型
- Author:
Li SU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Induce;
Cell differentiation;
Coculture
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the tansdifferentiation of bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte in a coculture system with cardiomyocytes.Methods:Newly born Wistar Rats' cardiomyocytes were isolated and cultured and the cells were labeled with DAPI.Prepared MSCs were planted into the cardiomyocytes dishes to establish a co-culture system.Supernatant liquid of the cardiomyocytes or fibroblast was added the MSCs(non-labeled) dishes as the control. After one week of coculture,fluorescence microscope observation and immunocytochemical staining with cTnT was performed to evaluate the phenotype changes in non-labeled MSCs.Results:MSCs and cardiomyocytes were connect with each other by net in culture plate after a few days of coculture;some non-labeled cells were positively stained by cTnT antibody while the MSCs cultured with cardiomyocytes or fibroblast supernatant liquid were stained negatively.Conclusion:To obtain cardiomyocyte phenotype in vitro,direct contact of MSCs with cardiomyocytes is essential.The signaling molecules induce MSCs' transformation into cardiomyocytes are likely some membrane proteins on the surface of cardiomyocytes.
