The mechanism of ~(131)I-GM-CSF inducing apoptosis of HL-60/ADM cells
- VernacularTitle:~(131)I-GM-CSF诱导HL-60/ADM细胞凋亡机制研究
- Author:
Ping ZHANG
- Publication Type:Journal Article
- Keywords:
~(131)I-GM-CSF;
Cell apoptosis;
Targeted radiopharmaceutic therapy;
Mitochondrial transmembrane potential
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of ~(131)I-GM-CSF inducing apoptosis of HL-60/ADM cells.Methods:An in vitro radioimmunotherapy(RAIT) model was employed.Data were collected from transmission electrion microscopy,flow cytometric analysis and immunocytochemistry assay.Results:After exposure to ~(131)I-GM-CSF,HL-60/ADM cells were induced to apoptosis.The cell mitochondrial transmembrane potential(MTP) reduced and HL-60/ADM cells appeared the classical apoptotic morphology.The Bax was up regulated,while Bcl-2 and Bcl-xl was decreased.Conclusion:~(131)I-GM-CSF can induce HL-60/ADM apoptosis through opening the mitochondrial permeability transition pore and reducing MTP,which suggests that ~(131)I-GM-CSF may be available in therapy of AML.
