Expression and significance of heme oxygenase-1 in delayed cardiac xenograft rejection
- VernacularTitle:HO-1在延迟性异种心脏移植排斥反应中的表达及意义
- Author:
Shengchun LIU
- Publication Type:Journal Article
- Keywords:
Heme oxygenase-1;
Delayed xenograft rejection;
Heart
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and significance of heme oxygenase-1(HO-1) in delayed cardiac xenograft rejection in NIH-Wistar.Methods:The model of cervical heterotopic heart transplantation in NIH-Wistar was established.The hearts were harvested 1,6,12,24,48 as well as 72 hours before and after transplantion respectively.HO-1mRNA and HO-1 protein were examined by RT-PCR and Western Blot,HO-1 enzymatic activity was examined.The survival time of transplanted heart,in which HO-1 was induced by cobalt protoporphyrin(CoPP),was compared with the control.Results:HO-1 was expressed in transplanted heart.The expression of HO-1mRNA(t=2.5170,P
