Effect of resistin on activity of glucoskinase and phosphoenolpyruvate carboxykinase in cultured liver cells
- VernacularTitle:抵抗素对肝细胞葡萄糖激酶和磷酸烯醇式丙酮酸激酶活性的影响
- Author:
Chaolin LI
- Publication Type:Journal Article
- Keywords:
Liver cells;
Glucoskinase;
Phosphoemolpyruvate carboxykinase;
Resistin
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of resistin on activity of glucoskinase(GK) and phosphoenolpyruvate carboxykinase(PEPCK) in cultured rat liver cells.Methods:Rat liver cells were incubated with resistin of different doses(ranging from 10nM/L to 100nM/L).Controled liver cells were invubated without resistin.Glucose-6-phosphate dehydrogenase couple chromatometry and lactate dehydrogenase couple chromatometry were used to determine activity of GK and PEPCK.Results:Compared with the control group,treated with resistin of 10nM/L and 50nM/L for 24h had no significant effect on activity of GK,but activity of PKPCK increased significantly(P
