The effect of methylmercury on the epithelial apoptosis of fetal mice intestine in vitro
- VernacularTitle:甲基汞影响小鼠胚胎肠上皮凋亡的体外实验
- Author:
Haiying ZHANG
- Publication Type:Journal Article
- Keywords:
Methylmercury chloride;
Fetal mouse;
Intestinal epithelium;
Apoptosis
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of methylmercury chloride(MMC) on the apoptosis and the expression of apoptosis-associated proteins in the intestinal epithelium of fetal mice.Methods:The duodenum and colon were taken from E13.5d and E14.5d fetal mice respectively,and cultured with different doses of MMC(0 ?g/L,200?g/L,400?g/L,800?g/L) for 24h.The samples were sliced into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies(NAB) in the fetal intestinal epithelium with stereological method.The others were stained with the immunohistochemical method to observe the expression of apoptosis-associated proteins Bcl-2,Bax,P53 and c-Fos.Results:①In experimental groups,all the NAB in the intestinal epithelium were higher than that in control groups(P
