Content determination of Puerarin in Xinkeshu Capsule by HPLC
- VernacularTitle:心可舒胶囊中葛根素含量的HPLC测定法
- Author:
Yong CHEN
- Publication Type:Journal Article
- Keywords:
Xinkeshu capsules;
Puerarin;
HPLC
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a HPLC method for the content determination of Puerarin in Xinkeshu capsule.Methods:The chromatography condition included C18 silicane-bonded silica gel column(250mm?4.6mm,5?m),MeOH-1.5%HAc-CHCI_3(25:74:1) mixture as the mobile phase,detection wavelength at 250nm,1.1ml/min of flow rate.Results:Puerarin was completely separated and no interference was found. The average recovery was 99.2%,RSD was 0.87%(n=5).Conclusion:The method is accurate,quick,sensitive and replicable,which is suitable for the quality control of Xinkeshu capsule.
