Inoculation of Lewis lung carcinoma cells enhances formalin-induced pain behavior and spinal Fos expression in mice.
10.4142/jvs.2017.18.3.267
- Author:
Jae Gyun CHOI
1
;
Jae Min KIM
;
Dong Wook KANG
;
Jung Wan CHOI
;
Jin Bong PARK
;
Seong Hun AHN
;
Yeonhee RYU
;
Hyun Woo KIM
Author Information
1. Department of Physiology and Medical Science and Brain Research Institute, College of Medicine, Chungnam National University, Daejeon 35015, Korea. kim0827@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Fos;
Lewis lung carcinoma;
formalin;
lung neoplasms;
pain
- MeSH:
Animals;
Carcinoma, Lewis Lung*;
Formaldehyde;
Humans;
Ibuprofen;
Incidence;
Lung Neoplasms;
Mice*;
Models, Animal;
Sensation
- From:Journal of Veterinary Science
2017;18(3):267-272
- CountryRepublic of Korea
- Language:English
-
Abstract:
The incidence of lung cancer has rapidly increased and cancer patients at a later cancer stage frequently suffer from unbearable cancer-associated pain. However, the pathophysiology of lung cancer pain has not been fully described due to a lack of appropriate animal models. This study was designed to determine the effect of Lewis lung carcinoma (LLC) cell inoculation on formalin-induced pain behavior and spinal Fos expression in C57BL/6 mice. LLC cells (1.5 × 10⁵, 2.5 × 10⁵, 3.0 × 10⁵ or 5.0 × 10⁵) were inoculated into back or peri-sciatic nerve areas. Back area inoculation was adopted to determine the effect of cancer cell circulating factors and the peri-sciatic nerve area was used to evaluate the possible effects of cancer cell contacting and circulating factors on formalin-induced pain. At postinoculation day 7, LLC cell (5.0 × 10⁵) inoculations in both back and peri-sciatic nerve area significantly increased formalin-induced paw-licking time and spinal Fos expression over those in cell-media-inoculated (control) mice. Enhanced pain behavior and spinal Fos expression were significantly suppressed by ibuprofen pretreatment (250 mg/kg). The results of this study suggest that LLC cell circulating factors and inflammatory responses may be critical in enhancing pain sensation in the early stage of lung cancer cell inoculation.
