Components identification and extraction optimization of Shuangshen Prescription
- VernacularTitle:双参复方成分鉴定与提取工艺优化
- Author:
Xiangdong LIU
;
Qionglin LIANG
;
Guoan LUO
;
Yiming WANG
- Publication Type:Journal Article
- Keywords:
Shuangshen Prescription;
uniform Design;
liquid chromatography-mass spectrometry (LC-MS);
component identification;
extraction
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify chemical components in Shuangshen Prescription by Liquid Chromatography-Mass Spectrometry (LC-MS), and optimize the extraction by uniform design (UD). METHODS: UD(U6(6^2)) was used to design the experiments of extraction, and LC-ESI-MS/MS was used to identify the components by MS1 and MS2. Meanwhile, we chose the extracted ion chromatography (EIC) area of each compound for applying UD analysis to optimize the factors influencing the extracration rate of Shuangshen Prescription, i.e. the amount of water added and the extracting time. RESULTS: Eight ginsenosides and six water-soluble components of salviae were identified in Shuangshen Prescription by LC/MS/MS. The extraction rate which was more than 90% for each of those 14 components, was highest under the condition of adding 25 times of water and extracting for 35 min. CONCLUSION: The study offered thc essential basis for further development and application of Shuangshen Prescription.
