Determination of curcumol and germacrone in Zedoary Turmeric Oil and Glucose Injections by RP-HPLC
- VernacularTitle:反相HPLC法测定莪术油葡萄糖注射液中莪术醇和吉马酮的含量
- Author:
Zhaogang HUANG
;
Shaoping LI
;
Jun LI
;
Quan XIA
;
Ping ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Zedoary Turmeric Oil and Glucose Injections;
curcumol;
germacrone
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC method for determination of curcumol and germacrone in Zedoary Turmeric Oil and Glucose Injections. METHODS: RP-HPLC was applied for quantitative analysis of curcumol and germacrone. Hypersil ODS2 column (250 mm? 4.6 mm, 5 ?m) and gradient elution was used. Mobile phase A was acetonitrile and mobile phase B was water. Phase A time-percentage composition was as follows:0-3 min,45%;3-30 min,45%→65%;30-38 min, 65%; 38-45 min, 65%→90%;45-55 min,90%→100%;55-65 min,100%. The flow rate was at 1.0 mL?min -1 . Diode array detector was set at 214 nm and column temperature was at 25 ?C. RESULTS: The calibration curves of curcumol and germacrone were linear in the range of 0.26 - 6.5 ?g(r= 0.999 9 ) and 0.112 5 - 2.812 5 ?g(r=1), The average recoveries of them were 102.1% and 98.8% (n=5), respectively. CONCLUSION: Curcumol and germacrone can be determined by the method, and this will improve the quality control of Zedoary Turmeric Oil and Glucose Injections.
