Analysis and quantitative determination of fatty acids in Hericium Erinaceus polysaccharide
- VernacularTitle:猴头多糖中脂肪酸成分分析及含量测定
- Author:
Yongjie YANG
;
Ruizhi JIANG
;
Yinghong CHEN
;
Qipin GAO
- Publication Type:Journal Article
- Keywords:
Hericium Erinaceus;
Polysaccharide;
fatty acid
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To assay fatty acids in Hericium Erinaceus polysaccharide by GC for establishing a method for quantitative determination of fatty acids. METHODS: The kinds of fatty acids were detected by GC-MS. The fatty acids contents were determined by GC: The sample was dissolved in sulphuric acid-methanol (1∶20) containing 0.5 mg heptadecanoic acid methyl ester and kept at 80?C for 3 hr. Then, the solution was extracted by hexamethylene four times and diluted to 1 mL. The extraction (0.4 ?L) was injected on a DB-1 column, using nitrogen as the carrier gas. The temperature was raised from 60?C to 200?C at the rate of 10?C?min -1 , and subsequently sustained for 30 min. The injection port and detector temperature were both at 250?C. RESULTS: The fatty acids were separated completely. The linear ranges were 0.4-3.6 mg?mL -1 . RSD97%. CONCLUSION: The fatty acids can be determined accurately by this method.
