Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus.
10.4142/jvs.2017.18.3.307
- Author:
Huawei LI
1
;
Jifei YANG
;
Dengke BAO
;
Jie HOU
;
Yubao ZHI
;
Yanyan YANG
;
Pengchao JI
;
Enmin ZHOU
;
Songlin QIAO
;
Gaiping ZHANG
Author Information
1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China. zhanggaiping2003@163.com
- Publication Type:Original Article
- Keywords:
enzyme-linked immunosorbent assay;
immunochromatography;
porcine reproductive and respiratory syndrome
- MeSH:
Antibodies*;
Collodion;
Colloids;
Diagnostic Tests, Routine;
Enzyme-Linked Immunosorbent Assay;
Gold Colloid;
Immunochromatography;
Membranes;
Porcine Reproductive and Respiratory Syndrome*;
Porcine respiratory and reproductive syndrome virus*;
Sensitivity and Specificity;
Staphylococcal Protein A;
Swine
- From:Journal of Veterinary Science
2017;18(3):307-316
- CountryRepublic of Korea
- Language:English
-
Abstract:
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.
