EFFECTS OF GANODERMA SPORE ON SURVIVAL AND EXPRESSION OF NT-3 AND NOS OF SPINAL MOTOR NEURON FOLLOWING VENTRAL ROOT CUT IN THE RAT
- VernacularTitle:灵芝孢子对大鼠脊神经腹根切断后脊髓运动神经元存活及其NT-3、NOS表达的影响
- Author:
Wei ZHANG
;
Yuansan ZENG
- Publication Type:Journal Article
- Keywords:
Ganoderma spore;
Ventral root cut;
Motor neuron;
Neurotrophin_3(NT_3);
Nitric oxide synthase(NOS);
Neuronal survival;
Immunohistochemistry;
In situ hybridization;
Rat
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Ganoderma spore(germination activated Ganoderma spore) on survival and expression of neurotrophin_3(NT_3) and nitric oxide synthase(NOS) of injured motor neurons in rat spinal cord. Methods Different dosages of Gandoerma spore were irrigated into the rat's stomach respectively in experimental groups after oneside ventral root cut.The survival ratio of injured motor neurons was counted.NT_3 expression of surviving motor neurons was measured by immunohistochemistry and in situ hybridization methods and NOS activity was measured by enzymo_histochemistry method. Results Thirty_five days after ventral root cut,the survival ratio of motor neurons was 47.32% in control group,and those were 67.11%,72.67% and 81.67% respectively in low,medial and high dosage Ganoderma spore groups.Expressions of NT_3 and NOS of surviving motor neurons in high dosage Ganoderma spore group were higher than those in control group.Conclusion Ganoderma spore may promote the survival of injured spinal motor neurons,and survival ratio of injured motor neurons is related to the dosages of Ganoderma spore.Ganoderma spore may also enhance the expressions of NT_3 and NOS of surviving spinal motor neurons.
