EXPRESSION OF VEGFR-3 IN LYMPHATIC ENDOTHELIUM OF THE SKIN IN RAT EMBRYOS
- VernacularTitle:大鼠胚胎皮肤淋巴管血管内皮生长因子受体-3的表达
- Author:
Jing MA
;
Linghui ZHAO
;
Baoquan LIU
;
Yafang ZHANG
;
Shucai YANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor receptor-3(VEGFR_3);
Lymphatic vessels;
Immunohistochemistry;
Skin;
Embryo
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the expression patterns of the vascular endothelial growth factor receptor 3(VEGFR-3) at the protein and mRNA level,and to detect the biological function of VEGFR-3 in the lymphangiogenesis of the embryos. Methods A total of 61 specimens of skin were investigated by immunohistochemical staining with a polyclonal antibody against VEGFR-3 in embryo 15-day-old(E15) and in embryo 21-day-old(E21).The expression of VEGFR-3 mRNA was studied in situ hybridization. Results The positive expression of VEGFR-3 can be seen in lymphatic vessels of the embryonic skin.The occurrences of VEGFR-3 protein in lymphatic vessels in E15 and in E21 were 38.71%(12/31) and 73.33%(22/30) respectively,the expression level of VEGFR-3 protein in E21 was significantly higher than that in E15(?~2=7.408,P
