EFFECT OF HEROIN DEPENDENCE ON EXPRESSION AND FUNCTION OF GLUCAGON IN ISLET A CELLS OF RATS
- VernacularTitle:海洛因依赖对大鼠胰岛A细胞胰高血糖素表达和功能的影响
- Author:
Jing HAN
;
Wenmei LIANG
- Publication Type:Journal Article
- Keywords:
Islet A cells;
Glucagon;
Heroin dependence;
Immunohistochemistry;
Image analysis;
Radioimmunoassay;
Rat
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of heroin dependence on the expression and the function of glucagon in islet A cells of rats.Methods The changes of numerical density on area of A cells and level of plasma glucagon of heroin-dependent rats were investigated by means of the immunohistochemical SABC method,image analysis and radioimmunoassay(RIA).Results As compared with SCG and NCG,it was observed that the number of Glu-IR positive cells increased and the intensity of immunohistochemical staining enhanced in the heroin-dependent groups.The result of image analysis showed that the numerical density on area of A cell apparently increased in the 38-day heroin-dependent group(P
