Effect of phosphorus deficiency on erythrocytic morphology and function in cows.
10.4142/jvs.2017.18.3.333
- Author:
Ziwei ZHANG
1
;
Mingyu BI
;
Jie YANG
;
Haidong YAO
;
Zhonghua LIU
;
Shiwen XU
Author Information
1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. liu086@126.com
- Publication Type:Original Article
- Keywords:
cow;
erythrocyte;
function;
morphology;
phosphorus deficiency
- MeSH:
Alanine Transaminase;
Aspartate Aminotransferases;
Bilirubin;
Diet;
Erythrocyte Indices;
Erythrocyte Membrane;
Erythrocytes;
Glutathione Peroxidase;
Malondialdehyde;
Membrane Fluidity;
Phosphorus*;
Superoxide Dismutase
- From:Journal of Veterinary Science
2017;18(3):333-340
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to evaluate the influence of phosphorus (P) deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg dry matter [DM]) or a control diet (0.36% P/kg DM). Red blood cell (RBC) indices results showed RBC and mean corpuscular hemoglobin decreased while mean corpuscular volume increased significantly (p < 0.05) in P-deficient cows. Erythrocyte morphology showed erythrocyte destruction in P-deficient cows. Erythrocytes' functional characteristics results showed total bilirubin and indirect bilirubin concentrations and aspartate transaminase and alanine transaminase activity levels in the serum of P-deficient cows were significantly higher than those in control diet-fed cows. Activities of superoxide dismutase and glutathione peroxidase in erythrocytes were lower, while the malondialdehyde content was greater, in P-deficient cows than in control diet-fed cows. Na⁺/K⁺-ATPase and Mg²⁺-ATPase activities were lower in P-deficient cows than in control diet-fed cows; however, Ca²⁺-ATPase activity was not significantly different. The phospholipid composition of the erythrocyte membrane changed and membrane fluidity rigidified in P-deficient cows. The results indicate that P deficiency might impair erythrocyte integrity and functional characteristics in cows.
