The association of hepatitis B virus precore/basic core promoter mutations with genotype and progression of liver disease
- VernacularTitle:慢性乙型肝炎病毒感染者病毒前C区和基本核心启动子区变异检测及意义
- Author:
Yuehui LIU
;
Jingjuan DING
;
Quan ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Basic core promoter;
Precore gene;
Mutation;
Genotype
- From:
Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study association of hepatitis B virus(HBV) precore (pre c)/basic core promoter(BCP) mutations with the genotype or the progression of liver disease. Methods The serum samples from 148 patients with HBV-relative diseases were collected, including 31 asymptomatic carriers, 32 with chronic hepatitis B (CHB), 40 with liver cirrhosis(LC) and 45 with hepatocellular carcinoma(HCC). The genes covering HBV pre c and BCP were amplified by nested polymerase chain reaction (nPCR). The PCR products were subjected to direct sequencing and the mutations in pre c 1896 and BCP 1762/1764 were determined by sequence analysis. HBV genotypes were also detected in the sera by restriction fragment length polymorphism based on S-gene PCR products. Results Of 148 serum samples of HBV, 128 were successfully genotyped and sequenced. There were 60 genotype B and 68 genotype C. The mutation in pre c (A1896) was significantly higher in genotype B than in genotype C (48.3% vs 29.34%, P
